Fluorometric Assay for DNA Polymerases and Reverse Transcriptase
نویسندگان
چکیده
منابع مشابه
Fluorometric Assay for DNA Polymerases and Reverse Transcriptase
We report a quick, easy and inexpensive fluorometric assay that measures the activity of replication enzymes using PicoGreen. The systems tested include replication of the natural template M13 Gori by E. coli DNA polymerase III holoenzyme and the replication of a synthetic homopolymer by human immunodeficiency virus reverse transcriptase. A direct comparison of the fluorometric assay with the ...
متن کاملFluorometric assay for DNA polymerases and reverse transcriptase.
We report a quick, easy and inexpensive fluorometric assay that measures the activity of replication enzymes using Pico-GreenTM. The systems tested include replication of the natural template M13 Gori by E. coli DNA polymerase III holoenzyme and the replication of a synthetic homopolymer by human immunodeficiency virus reverse transcriptase. A direct comparison of the fluorometric assay with th...
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ES cells. This phenomenon has been observed in our laboratory in a number of cases. Clones putatively identified as homologous recombinants by Southern analysis can be quickly checked for random insertion of extra copies of the targeting vector by PCR. Given the small difference in size between the two alleles and use of only two primers common to both WT and mutant DNA, the amplification rate ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96214st04